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human prostate cell lines pnt1a  (European Collection of Authenticated Cell Cultures)

 
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    European Collection of Authenticated Cell Cultures human prostate cell lines pnt1a
    Human Prostate Cell Lines Pnt1a, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pnt1a+cells/tsa+201+cells/pm39858418-43-0-18
    Average 90 stars, based on 1 article reviews
    human prostate cell lines pnt1a - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Lycopene enriched extra virgin olive oil: Biological activities and assessment of security profile on cells
    Article Snippet: .. The PNT1A cells (catalog no: 95012614) were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, England) and cultured in RPMI L. Marinaccio et al. Food Bioscience 60 (2024) 104466 1640 Medium (catalog no: A1049101) (Gibco, Waltham, MA, USA) supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin (Stefanucci et al., 2024). ..

    Food & Beverages:

    Article Title: Lycopene enriched extra virgin olive oil: Biological activities and assessment of security profile on cells
    Article Snippet: .. The PNT1A cells (catalog no: 95012614) were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, England) and cultured in RPMI L. Marinaccio et al. Food Bioscience 60 (2024) 104466 1640 Medium (catalog no: A1049101) (Gibco, Waltham, MA, USA) supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin (Stefanucci et al., 2024). ..

    Modification:

    Article Title: Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains.
    Article Snippet: Infection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host’s response to infection is strain-dependent.. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells.. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKVBR) using PCR array.

    other:

    Article Title: CYB561 supports the neuroendocrine phenotype in castration-resistant prostate cancer
    Article Snippet: PNT1A (Cat. No. 95012614) and LNCaP (Cat. No. 89110211) cells were purchased from the European Collection of Authenticated Cell Cultures, while 22Rv1 cells were obtained from the American Type Culture Collection (Cat. No. CRL-2505).



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    The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and <t>PNT1A</t> cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.
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    The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

    Journal: International Journal of Molecular Sciences

    Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

    doi: 10.3390/ijms24119513

    Figure Lengend Snippet: The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

    Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

    Journal: International Journal of Molecular Sciences

    Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

    doi: 10.3390/ijms24119513

    Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

    Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

    Techniques: Expressing

    AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

    Journal: International Journal of Molecular Sciences

    Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

    doi: 10.3390/ijms24119513

    Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

    Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

    Techniques: Flow Cytometry, Control, Western Blot, Expressing

    Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

    Journal: International Journal of Molecular Sciences

    Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

    doi: 10.3390/ijms24119513

    Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

    Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

    Techniques: Expressing

    AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

    doi: 10.3390/ijms24119513

    Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

    Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

    Techniques: Flow Cytometry, Expressing, Control